抗体分类 | Transcription Regulators |
货号 | HY-Ab-01357 |
名称 | Smad3 (phospho Thr179) Polyclonal Antibody |
种属 | Human;Mouse;Rat |
应用 | WB;IHC-p;IF(paraffin section);ELISA |
规格 | 50UL |
目录价(元) | 1350 |
规格 | 100UL |
目录价(元) | 2100 |
基因名称 | SMAD3 |
蛋白名称 | Mothers against decapentaplegic homolog 3 |
免疫原 | The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Thr179. AA range:145-194 |
特异性 | Phospho-Smad3 (T179) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at T179. |
组成 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
来源 | Polyclonal, Rabbit,IgG |
稀释 | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
纯化工艺 | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
浓度 | 1 mg/ml |
储存 | -20°C/1 year |
其他名称 | SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3 |
分子量 | 48081 |
实测条带 | 50 |
细胞定位 | Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969, PubMed:211454 |
组织表达 | Brain,Colon carcinoma,Esophagus tumor,Pancreas,Placenta,Spleen,Umbilical cord blood |
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